Term of Award

Summer 1998

Degree Name

Master of Science in Biology

Document Type and Release Option

Thesis (restricted to Georgia Southern)

Department

Department of Biology

Committee Chair

James H. Oliver, Jr.

Committee Member 1

Frank E. French

Committee Member 2

Wayne A. Krissinger

Abstract

Vitellin from Amblvomma americanum is a hemoglycolipophosphoprotein as shown by specific staining of polyacrylamide gels, carbohydrate and lipid analyses. The molecular weight was determined by gel permeation chromatography to be 360 kDa. Under reducing conditions (SDS-PAGE), the vitellin had 6 major polypeptides ranging from 70-210 kDa. The absorption maxima for the vitellin was 280 and 400 nm which is indicative of a protein containing heme. The predominant carbohydrates detected in A. americanum vitellin were mannose and N-acetylglucosamine as determined by gas chromatography. The neutral lipids detected in A. americanum vitellin were cholesterol, cholesteryl esters, fatty acids, triacylglycerides and diacylglycerides. The phospholipids associated with A. americanum were L-alpha-phosphatidylethanolamine and phosphatidylcholine. Amino acid analysis showed that the vitellin was rich in histidine, glutamic acid, valine, glycine and leucine, but threonine and tryosine concentrations were low.

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