Term of Award
Summer 1998
Degree Name
Master of Science in Biology
Document Type and Release Option
Thesis (restricted to Georgia Southern)
Department
Department of Biology
Committee Chair
James H. Oliver, Jr.
Committee Member 1
Frank E. French
Committee Member 2
Wayne A. Krissinger
Abstract
Vitellin from Amblvomma americanum is a hemoglycolipophosphoprotein as shown by specific staining of polyacrylamide gels, carbohydrate and lipid analyses. The molecular weight was determined by gel permeation chromatography to be 360 kDa. Under reducing conditions (SDS-PAGE), the vitellin had 6 major polypeptides ranging from 70-210 kDa. The absorption maxima for the vitellin was 280 and 400 nm which is indicative of a protein containing heme. The predominant carbohydrates detected in A. americanum vitellin were mannose and N-acetylglucosamine as determined by gas chromatography. The neutral lipids detected in A. americanum vitellin were cholesterol, cholesteryl esters, fatty acids, triacylglycerides and diacylglycerides. The phospholipids associated with A. americanum were L-alpha-phosphatidylethanolamine and phosphatidylcholine. Amino acid analysis showed that the vitellin was rich in histidine, glutamic acid, valine, glycine and leucine, but threonine and tryosine concentrations were low.
OCLC Number
1029252644
Catalog Permalink
https://galileo-georgiasouthern.primo.exlibrisgroup.com/permalink/01GALI_GASOUTH/1r4bu70/alma9916043092502950
Copyright
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Recommended Citation
Dudley, C. Kenneth Jr., "Characterization of Vitellin and Quantification of Vitellogenin in Amblyomma americanum (Acari: Ixodidae)" (1998). Legacy ETDs. 536.
https://digitalcommons.georgiasouthern.edu/etd_legacy/536
