Session Format
Conference Session (20 minutes)
Session Format
Presentation Session (45 minutes)
Target Audience
K12 Educators
Location
Room 212
Abstract for the conference program
In addition to its reproductive role, progesterone (P4) also has anti-inflammatory effects. While the precise mechanisms have never been clearly elucidated in RAW 246.7 cells, it seems logical to assume that this response is a consequence of the activation of the progesterone receptor (P4-R). However, it has been shown that in a rat model this anti-inflammatory effect is independent of the progesterone receptor. In this project, the aim was to characterize this response by assaying nitric oxide production from lipopolysaccharide-challenged RAW cells. To determine the involvement of the P4-R, cells were incubated in the presence and absence of RU-486 – a potent P4-R antagonist. Our results indicate that the anti-inflammatory response of progesterone was through activation of the P4-R as cells incubated in the antagonist show a reversal of this inhibitory effect of P4 as was seen in cells incubated in the absence of this ligand.
Proposal Track
Research Project
Proposal Track
T1: Teaching and Learning in the STEM Field
Start Date
3-3-2017 9:30 AM
End Date
3-3-2017 10:00 AM
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 License.
Recommended Citation
Brandon, Christopher I. Jr. and George, Bagie, "The Progesterone Receptor - To Be or Not to Be: The Anti-inflammatory Effects of Progesterone in RAW 264.7 Cells" (2017). Interdisciplinary STEM Teaching & Learning Conference (2012-2019). 5.
https://digitalcommons.georgiasouthern.edu/stem/2017/2017/5
The Progesterone Receptor - To Be or Not to Be: The Anti-inflammatory Effects of Progesterone in RAW 264.7 Cells
Room 212
In addition to its reproductive role, progesterone (P4) also has anti-inflammatory effects. While the precise mechanisms have never been clearly elucidated in RAW 246.7 cells, it seems logical to assume that this response is a consequence of the activation of the progesterone receptor (P4-R). However, it has been shown that in a rat model this anti-inflammatory effect is independent of the progesterone receptor. In this project, the aim was to characterize this response by assaying nitric oxide production from lipopolysaccharide-challenged RAW cells. To determine the involvement of the P4-R, cells were incubated in the presence and absence of RU-486 – a potent P4-R antagonist. Our results indicate that the anti-inflammatory response of progesterone was through activation of the P4-R as cells incubated in the antagonist show a reversal of this inhibitory effect of P4 as was seen in cells incubated in the absence of this ligand.
