A Laboratory Experience That Integrates Biochemistry and Instrumental Analysis
Abstract
We have selected the Green Fluorescent Protein (GFP) to develop a laboratory experience where students study key aspects of protein structure and function using fluorescence spectroscopy. In this experience, students learn how to: (1) purify GFP from transformed bacterial cells using hydrophobic interaction chromatography, (2) study GFP structure using open access databases (e.g., Protein Databank and UniProt), (3) determine the excitation and emission spectrum of GFP, and (4) examine the effect of pH and temperature on GFP function (i.e., fluorescence). Attendees will have the opportunity to examine the methodology that led to student experimental results and learn how the laboratory experience compares with other methods for conducting undergraduate experiments.
Location
Concourse
Recommended Citation
Flores, Rosa and Solá-Soto, Hilda, "A Laboratory Experience That Integrates Biochemistry and Instrumental Analysis " (2010). SoTL Commons Conference. 72.
https://digitalcommons.georgiasouthern.edu/sotlcommons/SoTL/2010/72
A Laboratory Experience That Integrates Biochemistry and Instrumental Analysis
Concourse
We have selected the Green Fluorescent Protein (GFP) to develop a laboratory experience where students study key aspects of protein structure and function using fluorescence spectroscopy. In this experience, students learn how to: (1) purify GFP from transformed bacterial cells using hydrophobic interaction chromatography, (2) study GFP structure using open access databases (e.g., Protein Databank and UniProt), (3) determine the excitation and emission spectrum of GFP, and (4) examine the effect of pH and temperature on GFP function (i.e., fluorescence). Attendees will have the opportunity to examine the methodology that led to student experimental results and learn how the laboratory experience compares with other methods for conducting undergraduate experiments.
