Location
Additional Presentations- College of Science and Mathematics
Document Type and Release Option
Thesis Presentation (Restricted to Georgia Southern)
Faculty Mentor
Dr. Robert Mans
Faculty Mentor Email
rmans@georgiasouthern.edu
Presentation Year
2021
Start Date
26-4-2021 12:00 AM
End Date
30-4-2021 12:00 AM
Keywords
Georgia Southern University, Honors Symposium, Presentation
Description
Zebrafish are gaining popularity as a model organism for the study of neurodegenerative diseases such as Alzheimer’s disease. However, shortcomings exist in current knowledge regarding the function of muscarinic acetylcholine receptors (mAChRs), a class of G-protein-coupled receptor, in zebrafish brain. Five types of mAChRs exist in the brains of mammals and zebrafish that contribute to cognitive processes. Extracellular signal-regulated kinase (ERK) is a protein required for learning and memory, and ERK activity is stimulated by phosphorylation downstream of mAChR activation. ERK dysfunction provides a potential link between mAChRs and disease pathogenesis. Previously, it has been shown that stimulation of mAChRs using the non-specific mAChR agonist oxo-m induces ERK phosphorylation in the zebrafish brain. The objective of the current study is to determine if the M1 subtype of mAChRs underlies the increase in phospho-ERK induced by oxo-m in the telencephalon, a structure required for learning and memory, in zebrafish. Zebrafish brains were extracted before treatment ex-vivo in oxygenated artificial cerebrospinal fluid containing oxo-m and pirenzepine, a M1 selective inhibitor. Following treatment, Western Blots were performed to determine the amount of phospho-ERK present in the telencephalon. The findings from this study will contribute to understanding the M1 receptor function in zebrafish.
Academic Unit
College of Science and Mathematics
Determining if M1 Receptor Underlies Phosphorylation of Extracellular Signal-Regulated Kinase Production in Adult Zebrafish Telencephalon
Additional Presentations- College of Science and Mathematics
Zebrafish are gaining popularity as a model organism for the study of neurodegenerative diseases such as Alzheimer’s disease. However, shortcomings exist in current knowledge regarding the function of muscarinic acetylcholine receptors (mAChRs), a class of G-protein-coupled receptor, in zebrafish brain. Five types of mAChRs exist in the brains of mammals and zebrafish that contribute to cognitive processes. Extracellular signal-regulated kinase (ERK) is a protein required for learning and memory, and ERK activity is stimulated by phosphorylation downstream of mAChR activation. ERK dysfunction provides a potential link between mAChRs and disease pathogenesis. Previously, it has been shown that stimulation of mAChRs using the non-specific mAChR agonist oxo-m induces ERK phosphorylation in the zebrafish brain. The objective of the current study is to determine if the M1 subtype of mAChRs underlies the increase in phospho-ERK induced by oxo-m in the telencephalon, a structure required for learning and memory, in zebrafish. Zebrafish brains were extracted before treatment ex-vivo in oxygenated artificial cerebrospinal fluid containing oxo-m and pirenzepine, a M1 selective inhibitor. Following treatment, Western Blots were performed to determine the amount of phospho-ERK present in the telencephalon. The findings from this study will contribute to understanding the M1 receptor function in zebrafish.
Comments
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