A Novel Method to Measure Exocytosis and Endocytosis Rates using Lipophilic Dye Fluorescence in Fission Yeast

Location

Poster Presentation- College of Science and Mathematics

Document Type and Release Option

Poster Presentation (Archived)

Faculty Mentor

Dr. Julien Berro and Dr. Yuan Ren of Yale University

Faculty Mentor Email

.

Presentation Year

2020

Start Date

4-2020 12:00 AM

End Date

5-2020 12:00 AM

Keywords

Georgia Southern University, Honors Symposium, Poster

Description

We investigated how fission yeast modify endocytosis and exocytosis by testing a high-throughput assay to measure the rate of exocytosis and endocytosis in live cells. The methodology produced consistent rates for exocytosis and endocytosis on short timescales, ~0-15 minutes. Future results could have important implications for other eukaryotes including humans.

Academic Unit

College of Science and Mathematics

Comments

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Apr 1st, 12:00 AM May 1st, 12:00 AM

A Novel Method to Measure Exocytosis and Endocytosis Rates using Lipophilic Dye Fluorescence in Fission Yeast

Poster Presentation- College of Science and Mathematics

We investigated how fission yeast modify endocytosis and exocytosis by testing a high-throughput assay to measure the rate of exocytosis and endocytosis in live cells. The methodology produced consistent rates for exocytosis and endocytosis on short timescales, ~0-15 minutes. Future results could have important implications for other eukaryotes including humans.