Document Type
Article
Publication Date
12-18-2018
Publication Title
BioMed Research International
DOI
10.1155/2018/2828143
ISSN
2314-6141
Abstract
To research carbonylated proteins and screen molecular targets in the rat striatum on regular aerobic exercise, male SpragueDawley rats (13 months old, n = 24) were randomly divided into middle-aged sedentary control (M-SED) and aerobic exercise (M-EX) groups (n = 12 each). Maximum oxygen consumption (VO2max) gradually increased from 50%–55% to 65%–70% for a total of 10 weeks. A total of 36 carbonylated proteins with modifed oxidative sites were identifed by Electrospray IonizationQuadrupole-Time of Flight-Mass Spectrometer (ESI-Q-TOF-MS), including 17 carbonylated proteins unique to the M-SED group, calcium/calmodulin-dependent protein kinase type II subunit beta (CaMKII�), and heterogeneous nuclear ribonucleoprotein A2/B1 (Hnrnpa2b1), among others, and 19 specifc to the M-EX group, ubiquitin carboxyl-terminal hydrolase isozyme L1 (UCHL1), and malic enzyme, among others. Regular aerobic exercise improved behavioral and stereological indicators, promoted normal apoptosis (P < 0.01), alleviated carbonylation of the CaMKII� and Hnrnpa2b1, but induced carbonylation of the UCH-L1, and signifcantly upregulated the expression levels of CaMKII�, CaMKII�, and Vdac1 (p < 0.01) and Hnrnpa2b1 and UCH-L1 (p < 0.01), as well as the phosphoinositide 3-kinase/protein kinase B/mammalian target of rapamycin pathways (PI3K/Akt/mTOR) pathway-related genes Akt and mTOR. Regular aerobic exercise for 10 weeks (incremental for the frst 6 weeks followed by constant loading for 4 weeks) enhanced carbonylation of CaMKII�, Hnrnpa2b1, and modulated apoptosis via activation of CaMK and phosphoinositide 3-kinase/protein kinase B/mTOR signaling. It also promoted normal apoptosis in the rat striatum, which may have protective efects in neurons.
Recommended Citation
Liu, Wenfeng, Li Li, Heyu Kuang, Yan Xia, Zhiyuan Wang, Shaopeng Liu, Dazhong Yin.
2018.
"Proteomic Profile of Carbonylated Proteins Screen Regulation of Apoptosis via CaMK Signaling in Response to Regular Aerobic Exercise."
BioMed Research International.
doi: 10.1155/2018/2828143 source: 10.1155/2018/2828143
https://digitalcommons.georgiasouthern.edu/health-kinesiology-facpubs/151
Comments
Copyright © 2018 Wenfeng Liu et al. Tis is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.