Term of Award

Spring 1991

Degree Name

Master of Science in Biology

Document Type and Release Option

Thesis (restricted to Georgia Southern)

Committee Chair

James H. Oliver, Jr.

Committee Member 1

Sara Neville Bennett

Committee Member 2

Wayne A. Krissinger

Abstract

Karyotypes stained with conventional Giemsa or orcein stain and with a C-banding procedure were compared among Ixodes dammini Spielman, Clifford, Piesman & Corwin, Ixodes scapularis Say, and Ixodes pacificus Cooley & Kohls. All three Ixodes species examined possessed 28 chromosomes, 26 autosomes and XX : XY sex chromosomes; the X chromosome was the largest element and the Y chromosome the smallest one in the karyotype. No significant difference among species (P>0.05) was noted in relative lengths of mitotic metaphase autosomes and sex chromosomes. C-bands of /. dammini, I. scapularis and I. pacificushad small or medium-size heterochromatic bands and were located mainly on the centromeric regions. Heterochromatin (C-bands) of autosomes of the three species was terminal, subterminal and submedian. The sex chromosome had a terminal C-band. Several differences were noted in C-banding. One difference was the short arm of chromosome 7 of I. dammini and I. scapularis which had interstitial C-bands, as opposed to I. pacificus with a terminal C-band. Some /. dammini had a light staining band and other specimens an absence of heterochromatic material on chromosomes 1, 2 and 5. The C-bands of chromosomes 2 and 10 were subterminal in I. dammini and /. scapularis while they were terminal in /. pacificus; chromosomes 6 and 11 were terminal in /. dammini and I. scapularis and subterminal in I. pacificus. C-banded chromosomes of the three Ixodes species indicate that I. dammini and /. scapularis are more closely related to each other than either is to I. pacificus.

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