Term of Award

Spring 1991

Degree Name

Master of Science in Biology

Document Type and Release Option

Thesis (restricted to Georgia Southern)

Department

Department of Biology

Committee Chair

James H. Oliver, Jr.

Committee Member 1

Sara Neville Bennett

Committee Member 2

Wayne A. Krissinger

Abstract

Karyotypes stained with conventional Giemsa or orcein stain and with a C-banding procedure were compared among Ixodes dammini Spielman, Clifford, Piesman & Corwin, Ixodes scapularis Say, and Ixodes pacificus Cooley & Kohls. All three Ixodes species examined possessed 28 chromosomes, 26 autosomes and XX : XY sex chromosomes; the X chromosome was the largest element and the Y chromosome the smallest one in the karyotype. No significant difference among species (P>0.05) was noted in relative lengths of mitotic metaphase autosomes and sex chromosomes. C-bands of /. dammini, I. scapularis and I. pacificushad small or medium-size heterochromatic bands and were located mainly on the centromeric regions. Heterochromatin (C-bands) of autosomes of the three species was terminal, subterminal and submedian. The sex chromosome had a terminal C-band. Several differences were noted in C-banding. One difference was the short arm of chromosome 7 of I. dammini and I. scapularis which had interstitial C-bands, as opposed to I. pacificus with a terminal C-band. Some /. dammini had a light staining band and other specimens an absence of heterochromatic material on chromosomes 1, 2 and 5. The C-bands of chromosomes 2 and 10 were subterminal in I. dammini and /. scapularis while they were terminal in /. pacificus; chromosomes 6 and 11 were terminal in /. dammini and I. scapularis and subterminal in I. pacificus. C-banded chromosomes of the three Ixodes species indicate that I. dammini and /. scapularis are more closely related to each other than either is to I. pacificus.

OCLC Number

1031712837

Copyright

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