Author

Can CaiFollow

Term of Award

Spring 2020

Degree Name

Master of Science, Applied Physical Science

Document Type and Release Option

Thesis (open access)

Copyright Statement / License for Reuse

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 License.

Department

Interdisciplinary

Committee Chair

Xiao-Jun Wang

Committee Member 1

Li Ma

Committee Member 2

Ji Wu

Abstract

In this work, nitroxide 2,2,6,6-tetramethylpiperdine 1-oxyl (Tempo), human serum albumin (HSA), CuSO4 and phosphate buffered saline (PBS) were used to synthesis the hybrid nanoflowers. Scanning electron microscopy (SEM), electron paramagnetic resonance (EPR), and Fourier-transform infrared spectroscopy (FTIR) were used for characterization of the nanoflowers.The SEM spectra illustrated the morphologies of the hybrid nanoflowers. The FTIR spectra corroborated the presence of PNA in the hybrid nanoflowers, while the EPR spectra confirmed the intermolecular interaction of nitroxides bound to the human serum albumin incorporated into the nanoflowers. The catalase mimetic activity and corresponding kinetic parameters of nanoflowers were determined using the H2O2 depletion and O2 evolution assays. The superoxide dismutase mimetic activity of the nanoflowers was determined by the xanthine oxidase/cytochrome c method. And the reusability of the hybrid nanoflowers was assessed via the H2O2 depletion assay and the xanthine oxidase/cytochrome c method. Kinetic analysis revealed that the reactions of the incorporated nanoflowers with H2O2 obey Michaelis–Menton kinetics and the nanoflowers possess a higher affinity for H2O2 than nature catalase. the hybrid nanoflowers with superior stability and desired ligand grafting sites will find widespread utilization in the medical sciences.

Research Data and Supplementary Material

No

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