Term of Award
Spring 2020
Degree Name
Master of Science, Applied Physical Science
Document Type and Release Option
Thesis (open access)
Copyright Statement / License for Reuse
This work is licensed under a Creative Commons Attribution 4.0 License.
Department
Interdisciplinary
Committee Chair
Xiao-Jun Wang
Committee Member 1
Li Ma
Committee Member 2
Ji Wu
Abstract
In this work, nitroxide 2,2,6,6-tetramethylpiperdine 1-oxyl (Tempo), human serum albumin (HSA), CuSO4 and phosphate buffered saline (PBS) were used to synthesis the hybrid nanoflowers. Scanning electron microscopy (SEM), electron paramagnetic resonance (EPR), and Fourier-transform infrared spectroscopy (FTIR) were used for characterization of the nanoflowers.The SEM spectra illustrated the morphologies of the hybrid nanoflowers. The FTIR spectra corroborated the presence of PNA in the hybrid nanoflowers, while the EPR spectra confirmed the intermolecular interaction of nitroxides bound to the human serum albumin incorporated into the nanoflowers. The catalase mimetic activity and corresponding kinetic parameters of nanoflowers were determined using the H2O2 depletion and O2 evolution assays. The superoxide dismutase mimetic activity of the nanoflowers was determined by the xanthine oxidase/cytochrome c method. And the reusability of the hybrid nanoflowers was assessed via the H2O2 depletion assay and the xanthine oxidase/cytochrome c method. Kinetic analysis revealed that the reactions of the incorporated nanoflowers with H2O2 obey Michaelis–Menton kinetics and the nanoflowers possess a higher affinity for H2O2 than nature catalase. the hybrid nanoflowers with superior stability and desired ligand grafting sites will find widespread utilization in the medical sciences.
OCLC Number
1158465575
Catalog Permalink
https://galileo-georgiasouthern.primo.exlibrisgroup.com/permalink/01GALI_GASOUTH/1fi10pa/alma9916375149802950
Recommended Citation
Cai, Can, "Nitroxide Human Serum Albumin Incorporated Nanoflowers With Dual Enzyme-Like Activities" (2020). Electronic Theses and Dissertations. 2099.
https://digitalcommons.georgiasouthern.edu/etd/2099
Research Data and Supplementary Material
No