Document Type
Article
Publication Date
9-26-2003
Publication Title
Journal of Biological Chemistry
DOI
10.1074/jbc.M305126200
ISSN
1083-351X
Abstract
In Na,K-ATPase membrane preparations from shark rectal glands, we have previously identified an FXYD domain-containing protein, phospholemman-like protein from shark, PLMS. This protein was shown to associate and modulate shark Na,K-ATPase activity in vitro. Here we describe the complete coding sequence, expression, and cellular localization of PLMS in the rectal gland of the shark Squalus acanthias. The mature protein contained 74 amino acids, including the N-terminal FXYD motif and a C-terminal protein kinase multisite phosphorylation motif. The sequence is preceded by a 20 amino acid candidate cleavable signal sequence. Immunogold labeling of the Na,K-ATPase α-subunit and PLMS showed the presence of α and PLMS in the basolateral membranes of the rectal gland cells and suggested their partial colocalization. Furthermore, through controlled proteolysis, the C terminus of PLMS containing the protein kinase phosphorylation domain can be specifically cleaved. Removal of this domain resulted in stimulation of maximal Na,K-ATPase activity, as well as several partial reactions. Both the E1∼P → E2-P reaction, which is partially rate-limiting in shark, and the K+ deocclusion reaction, E2(K) → E1, are accelerated. The latter may explain the finding that the apparent Na+ affinity was increased by the specific C-terminal PLMS truncation. Thus, these data are consistent with a model where interaction of the phosphorylation domain of PLMS with the Na,K-ATPase α-subunit is important for the modulation of shark Na,K-ATPase activity.
Recommended Citation
Mahmmoud, Yasser A., Gordon Cramb, Arvid B. Maunsbach, Christopher P. Cutler, Lara Meischke, Flemming Cornelius.
2003.
"Regulation of Na,K-ATPase by PLMS, the Phospholemman-Like Protein From Shark: Molecular Cloning, Expression, Cellular Distribution and Functional Effects of PLMS."
Journal of Biological Chemistry, 278 (39): 37427-37428.
doi: 10.1074/jbc.M305126200 source: https://www.sciencedirect.com/science/article/pii/S0021925820833031?via%3Dihub
https://digitalcommons.georgiasouthern.edu/biology-facpubs/67
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Comments
This research was originally published in the Journal of Biological Chemistry. Mahmmoud, et al. Regulation of Na,K-ATPase by PLMS, the Phospholemman-Like Protein From Shark: Molecular Cloning, Expression, Cellular Distribution and Functional Effects of PLMS. J. Biol. Chem. 2003; 278, 37427-37438. © 2003 by The American Society for Biochemistry and Molecular Biology, Inc.
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