Integrating Waveguide Biosensor
Document Type
Article
Publication Date
1-1-2002
Publication Title
Analytical Chemistry
DOI
10.1021/ac015607s
ISSN
0003-2700
Abstract
A capillary biosensor is demonstrated which uses the waveguiding properties of the capillary to integrate the signal over an increased surface area without simultaneously increasing the background noise from the detector. This biosensor achieves limits of detection of 30-50 pg/mL in immunoassays using a diode laser for excitation and a PMT for detection. This is approximately 2 orders of magnitude greater sensitivity than was achieved using the same immunoassay reagents in a fiber optic biosensor or a planar array biosensor. Two different approaches to using the capillaries as immunosensors are described, either of which could be adapted for multianalyte sensing.
Recommended Citation
Ligler, Francis S., Marc Breimer, Joel P. Golden, Delana A. Gajdosik-Nivens, James P. Dodson, Tiffanee M. Greene, Daniel P. Haders, Omowunmi A. Sadik.
2002.
"Integrating Waveguide Biosensor."
Analytical Chemistry, 74 (3): 713-719.
doi: 10.1021/ac015607s
https://digitalcommons.georgiasouthern.edu/biology-facpubs/185
