Proposal Title

A Laboratory Experience That Integrates Biochemistry and Instrumental Analysis

Proposal Abstract

We have selected the Green Fluorescent Protein (GFP) to develop a laboratory experience where students study key aspects of protein structure and function using fluorescence spectroscopy. In this experience, students learn how to: (1) purify GFP from transformed bacterial cells using hydrophobic interaction chromatography, (2) study GFP structure using open access databases (e.g., Protein Databank and UniProt), (3) determine the excitation and emission spectrum of GFP, and (4) examine the effect of pH and temperature on GFP function (i.e., fluorescence). Attendees will have the opportunity to examine the methodology that led to student experimental results and learn how the laboratory experience compares with other methods for conducting undergraduate experiments.

Location

Concourse

Publication Type and Release Option

Presentation (Open Access)

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Mar 11th, 4:00 PM Mar 11th, 5:45 PM

A Laboratory Experience That Integrates Biochemistry and Instrumental Analysis

Concourse

We have selected the Green Fluorescent Protein (GFP) to develop a laboratory experience where students study key aspects of protein structure and function using fluorescence spectroscopy. In this experience, students learn how to: (1) purify GFP from transformed bacterial cells using hydrophobic interaction chromatography, (2) study GFP structure using open access databases (e.g., Protein Databank and UniProt), (3) determine the excitation and emission spectrum of GFP, and (4) examine the effect of pH and temperature on GFP function (i.e., fluorescence). Attendees will have the opportunity to examine the methodology that led to student experimental results and learn how the laboratory experience compares with other methods for conducting undergraduate experiments.