Presentation Title

Examining the Effect of 2-Aminoanthracene Exposure in Sprague Dawley Dams from Gestation through the Postnatal Period

Location

Room 1909

Session Format

Paper Presentation

Research Area Topic:

Natural & Physical Sciences - Chemistry

Co-Presenters, Co- Authors, Co-Researchers, Mentors, or Faculty Advisors

Hunter Daniel#; Yau, Wilson§; Howerth, Elizabeth W§ and Gato, Worlanyo E#+

# Department of Chemistry, Georgia Southern University, Statesboro, GA 30458

§ Department of Pathology, College of Veterinary Medicine, The University of Georgia, Athens, GA 30602

Abstract

Background: The effect of 2-Aminoanthracene (2AA) on adipose tissue gene expression in pregnant Sprague Dawley rats was investigated. Adipocyte dysfunction may be a critical link between obesity and insulin resistance as a result of abnormal fat storage and mobilization. We have previously observed insulin-signaling related altered gene expression in animals exposed to 2AA. 2AA is an amino-substituted polycyclic aromatic hydrocarbon used in manufacturing dyes, chemical, inks, resins, and polyurethanes. 2AA is a known mutagen and carcinogen that occurs naturally and can be found in tobacco smoke and cooked foods. To examine insulin-dependent 2AA effects on the adipose tissue, nine timed pregnant dams were assigned into dose regimens of 0 mg/kg- (control-C), 50 mg/kg- (low dose-LD) and 100 mg/kg-diet (high dose-HD) 2AA. Dams were fed 2AA contaminated diet during the period of gestation and postpartum. The expression of key gene transcripts reported to be important in mediating inflammatory processes was examined via quantitative RT-PCR. Histologic examination of the adipose tissue (AT) was also carried out to understanding the anatomy of the AT due to 2AA exposure during gestation and postpartum.

Results: Body weight gain during gestation and postnatal period indicated no significant differences in animals. Examination of the adipose tissue (AT) for microscopic changes suggests no alterations between control and low dose animals. However, AT of the high dose animals is infiltrated by increased numbers of CD68+mononuclear cells (macrophages) and small numbers of eosinophils and mast cells, consistent with inflammation. In addition, analysis of the mRNA expression of cytokines and adipokines demonstrates the importance of inflammation in ATs dysfunction. For instance, TNFa, LEPTIN and IL-6 transcripts were relatively more expressed in the low dose animals than the high dose and control rats.

Conclusion: It appears the effects of 2AA on pregnant dams were more pronounced in the low dose group than the high dose group using differential gene expression levels, possibly indicating increased susceptibility of rat offspring within this group to diabetic-type conditions.

Keywords

2-Aminoanthracene, Adipose tissue (AT), Insulin sensitivity, CD68, TNFα, Inflammatory response

Presentation Type and Release Option

Presentation (Open Access)

Start Date

4-24-2015 4:00 PM

End Date

4-24-2015 5:00 PM

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Apr 24th, 4:00 PM Apr 24th, 5:00 PM

Examining the Effect of 2-Aminoanthracene Exposure in Sprague Dawley Dams from Gestation through the Postnatal Period

Room 1909

Background: The effect of 2-Aminoanthracene (2AA) on adipose tissue gene expression in pregnant Sprague Dawley rats was investigated. Adipocyte dysfunction may be a critical link between obesity and insulin resistance as a result of abnormal fat storage and mobilization. We have previously observed insulin-signaling related altered gene expression in animals exposed to 2AA. 2AA is an amino-substituted polycyclic aromatic hydrocarbon used in manufacturing dyes, chemical, inks, resins, and polyurethanes. 2AA is a known mutagen and carcinogen that occurs naturally and can be found in tobacco smoke and cooked foods. To examine insulin-dependent 2AA effects on the adipose tissue, nine timed pregnant dams were assigned into dose regimens of 0 mg/kg- (control-C), 50 mg/kg- (low dose-LD) and 100 mg/kg-diet (high dose-HD) 2AA. Dams were fed 2AA contaminated diet during the period of gestation and postpartum. The expression of key gene transcripts reported to be important in mediating inflammatory processes was examined via quantitative RT-PCR. Histologic examination of the adipose tissue (AT) was also carried out to understanding the anatomy of the AT due to 2AA exposure during gestation and postpartum.

Results: Body weight gain during gestation and postnatal period indicated no significant differences in animals. Examination of the adipose tissue (AT) for microscopic changes suggests no alterations between control and low dose animals. However, AT of the high dose animals is infiltrated by increased numbers of CD68+mononuclear cells (macrophages) and small numbers of eosinophils and mast cells, consistent with inflammation. In addition, analysis of the mRNA expression of cytokines and adipokines demonstrates the importance of inflammation in ATs dysfunction. For instance, TNFa, LEPTIN and IL-6 transcripts were relatively more expressed in the low dose animals than the high dose and control rats.

Conclusion: It appears the effects of 2AA on pregnant dams were more pronounced in the low dose group than the high dose group using differential gene expression levels, possibly indicating increased susceptibility of rat offspring within this group to diabetic-type conditions.