Presentation Title

Microbial Source Tracking at a Tidal River Beach

Location

Atrium

Session Format

Poster Presentation

Research Area Topic:

Natural & Physical Sciences - Environmental Sciences & Sustainability

Co-Presenters, Co- Authors, Co-Researchers, Mentors, or Faculty Advisors

Asheley Poole, Bushra Shah, Asli Aslan

Abstract

Kings Ferry Beach is located along the Ogeechee River and is a popular site for recreational activities in Georgia’s coastal waterways. The Georgia Department of Natural Resources (GADNR) has issued a permanent advisory at this beach due to persistently high numbers of enterococci. In this study, water samples were collected monthly from 14 locations both in and around the beach site. Samples were tested using the same method that the GADNR using for beach monitoring (EPA Method 1600) and a new molecular method (HF183 human marker) using quantitative polymerase chain reaction (qPCR) to detect sewage as the source of pollution in water. Preliminary findings are based on samples obtained October, November, and December of 2014. Statistical analysis was performed using IBM SPSS version 21.

Among 42 samples analyzed from October to December 2014, 66.67% (n=28) of the samples exceeded USEPA beach action value for enterococci (60 CFU/100mL). The enterococci concentrations ranged from 20 to 507 CFU/100 ml. Highest numbers of human marker (HF183) was detected in front of a trailer park (131 CCE/100 ml) and 118 (CCE/100 ml) in front of a storm drain respectively. These points were both located the beach. A regression analysis was performed to compare Enterococci and HF183 concentrations. Results showed that there is a significant difference between Enterococci and HF183 (p

Keywords

Microbial source tracking, Sewage, Quantitative polymerase chain reaction

Presentation Type and Release Option

Presentation (Open Access)

Start Date

4-24-2015 2:45 PM

End Date

4-24-2015 4:00 PM

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Apr 24th, 2:45 PM Apr 24th, 4:00 PM

Microbial Source Tracking at a Tidal River Beach

Atrium

Kings Ferry Beach is located along the Ogeechee River and is a popular site for recreational activities in Georgia’s coastal waterways. The Georgia Department of Natural Resources (GADNR) has issued a permanent advisory at this beach due to persistently high numbers of enterococci. In this study, water samples were collected monthly from 14 locations both in and around the beach site. Samples were tested using the same method that the GADNR using for beach monitoring (EPA Method 1600) and a new molecular method (HF183 human marker) using quantitative polymerase chain reaction (qPCR) to detect sewage as the source of pollution in water. Preliminary findings are based on samples obtained October, November, and December of 2014. Statistical analysis was performed using IBM SPSS version 21.

Among 42 samples analyzed from October to December 2014, 66.67% (n=28) of the samples exceeded USEPA beach action value for enterococci (60 CFU/100mL). The enterococci concentrations ranged from 20 to 507 CFU/100 ml. Highest numbers of human marker (HF183) was detected in front of a trailer park (131 CCE/100 ml) and 118 (CCE/100 ml) in front of a storm drain respectively. These points were both located the beach. A regression analysis was performed to compare Enterococci and HF183 concentrations. Results showed that there is a significant difference between Enterococci and HF183 (p