Detecting the Presence of Aquaporin 4b in the American eel (Anguilla rostrata) in kidney structures and cloning and sequencing a fragment of Aquaporin 0 from the dogfish shark (Squalus acanthias)

Name

Jordan J. Garrett, Georgia Southern UniversityFollow

Publication Date

4-29-2014

Major

Biology (B.S.B.)

Document Type and Release Option

Thesis (restricted to Georgia Southern)

Faculty Mentor

Dr. Christopher Cutler

Abstract

Abstract

Aquaporins are thought to be involved in water absorption in the intestinal tract, yet little is known about its function or of its presence of the protein in other aquatic organs. AQP4 is found at highest levels in the brain, heart, and kidney. Eel aquaporin 4b is an ancient duplication of aquaporin 4. Localization of the AQP4b protein in eel kidney was performed using an antibody and immunohistochemistry, and this detected the presence of these proteins in unidentified ring-like structures and along tracts. Although the exact cells displaying immunofluorescence with the AQP4b antibody were not been determined, possibilities to be explored in future include oligodendrocytes or chromaffin cells. Amplification and cloning of the 3' end of the aquaporin 0 (AQP0) gene from the dogfish shark, Squalus acanthias was completed using RACE PCR. AQP 0 3’ cDNA fragments were processed and analyzed, and were then combined with those obtained by others, leading to the complete nucleotide and amino acid sequence of the dogfish shark AQP0 water channel cDNA and its respective protein.

Recommended Citation

Garrett, Jordan J., "Detecting the Presence of Aquaporin 4b in the American eel (Anguilla rostrata) in kidney structures and cloning and sequencing a fragment of Aquaporin 0 from the dogfish shark (Squalus acanthias)" (2014). Honors College Theses. 37.
https://digitalcommons.georgiasouthern.edu/honors-theses/37