Honors College Theses

Publication Date

4-29-2014

Major

Biology (B.S.B.)

Release Option

Restricted to Georgia Southern

Faculty Mentor

Christopher Cutler

Abstract

Localization of AQP3 and AQPe: Aquaporin are expressed in the kidneys of American eel (Anguilla rostrata) and transport water across epithelial tissue of the tubules and collecting ducts. The study utilized immunohistochemical localization and co-localization with other isoforms of aquaporin in order to investigate expression of AQP3 and AQPe in the kidneys of freshwater- and saltwater-acclimated eels. AQP3 could possibly be localized in the distal tubule and play a role in water re-absorption. AQPe exhibited lateral and basolateral tubule staining, suggesting its role in preventing swelling and shrinkage of tubular cells.

AQP0 gene cloning: In collaboration with another student, the AQP0 gene cDNA for Squalus acanthias was cloned and sequenced. RT-PCR was utilized to determine expression levels of AQP0 mRNA in various tissues. AQP0 mRNA was found to be expressed in every tissue examined.

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