Immunohistochemical Localization of the Aquaporins AQPe and AQP3 in Kidneys of Freshwater and Saltwater-acclimated American Eel (Anguilla rostrata): Cloning of the 5' End of AQP0 Gene from the Dogfish Shark, (Squalus acanthias)
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Thesis (restricted to Georgia Southern)
Localization of AQP3 and AQPe: Aquaporin are expressed in the kidneys of American eel (Anguilla rostrata) and transport water across epithelial tissue of the tubules and collecting ducts. The study utilized immunohistochemical localization and co-localization with other isoforms of aquaporin in order to investigate expression of AQP3 and AQPe in the kidneys of freshwater- and saltwater-acclimated eels. AQP3 could possibly be localized in the distal tubule and play a role in water re-absorption. AQPe exhibited lateral and basolateral tubule staining, suggesting its role in preventing swelling and shrinkage of tubular cells.
AQP0 gene cloning: In collaboration with another student, the AQP0 gene cDNA for Squalus acanthias was cloned and sequenced. RT-PCR was utilized to determine expression levels of AQP0 mRNA in various tissues. AQP0 mRNA was found to be expressed in every tissue examined.
Ogden, Daniel D., "Immunohistochemical Localization of the Aquaporins AQPe and AQP3 in Kidneys of Freshwater and Saltwater-acclimated American Eel (Anguilla rostrata): Cloning of the 5' End of AQP0 Gene from the Dogfish Shark, (Squalus acanthias)" (2014). University Honors Program Theses. 35.