Honors College Theses

Publication Date



Biology (B.S.B.)

Document Type and Release Option

Thesis (restricted to Georgia Southern)

Faculty Mentor

Dr. Chris Cutler


Although the knowledge of ion transporters located in the renal tubules of fish has increased in recent years, there is still a deficit of information on the water transporters in fish. To address this problem, antibodies were created (or purchased) against ion transporters to act as markers of elasmobranch nephron segments. Immunohistochemistry was then conducted with those and antibodies against specific aquaporins (AQPs). AQP4 was found in both the bundle zone early distal tubule (EDT) and the sinus zone late distal tubule (LDT). In the sinus zone, Na-K-ATPase (NKA) co-localized with AQP4 and AQP15 in initial parts of the late distal tubule (LDT). The location of AQP3 in LDT somewhat overlapped with the other AQPs and NKA, but staining was stronger in later LDT regions.