Term of Award
Master of Science
Document Type and Release Option
Thesis (restricted to Georgia Southern)
Sara Neville Bennett
Committee Member 1
Wayne A. Krissinger
Committee Member 2
Genetic research was conducted using os-11 trp-1 double mutants in this study. Conidia from the double mutants were subjected to UV irradiation and os-11+ revertants were isolated. A total of 47 putative revertants were isolated in the study. Two of the putative revertants were crossed to double mutants. The cross Rev-2-4AX DA-2-68a exhibited a low viability at 8% and the second cross Rev-2-13XDA-60 had a viability of 22% for progeny. The revertants revealed the presence of the trp-1 a genetic marker. Recovery of os+ progeny indicated that the reversion was genetic.
Growth tests in the study measured osmotic sensitivity of the osmotic strains isolated in the Neurospora Genetics Laboratory at Georgia Southern University, os-8, os-9 (SS-462) os-9 (SS-788), os-10, and os-11, and compared their sensitivity to earlier reported strains os-1, os-2, os-4, and os-5.
On medium with NaCl as the osmoticum the mutants os-8, os-9 (SS-462), os-9 (SS-788) and os-1 failed to grow at .684 osmolality; os-5 failed to grow ax 1.37 osmolality; os-10, os-11, os-2 failed to grow at 1.71 osmolality.
On medium with KC1 as the osmoticum the mutants os-1 and os-10 failed to grow at 1.03 osmolality; os-2, os-5 and os-8 failed to grow at 1.37 osmalality. os-11 failed to grow at 2.05 osmalality and os-4 failed to grow at 2.74 osmolality The two alleles of os-9 were less sensitive. The mutant os-9 (SS-788) failed to grow at 3.42 osmolality. The mutant os-9 (SS-462) was not sensitive at the concentrations of KC1 tested and grew at 3.42 osmolality.
On medium with glucose as the osmoticum the mutants, os-1 and os-2 failed to grow at .684 osmolality. The mutants 05-5 and os-10 failed to grow at 1.03 osolality; os-8 and os-11 failed to grow at 2.05 osmolality. Both alleles, SS-462 and SS-788, of os-9 failed to grow at 3.42 osmolality.
Thus, the two alleles of os-9 were sensitive on media supplemented with NaCl, but failed to show sensitivity on media with KC1 or glucose, as the osmoticum. This suggests that the sensitivity of os-9 might be specific for NaCl.
To obtain a full copy of this work, please visit the campus of Georgia Southern University or request a copy via your institution's Interlibrary Loan (ILL) department. Authors and copyright holders, learn how you can make your work openly accessible online.
Adams, Dewayne Leon, "Characterization of Osmotic Sensitivity of os-9 and os-11 Mutants of Neurospora crassa" (2002). Legacy ETDs. 735.