Term of Award

Fall 1991

Degree Name

Master of Science in Biology

Document Type and Release Option

Thesis (restricted to Georgia Southern)

Department

Department of Biology

Committee Chair

Wayne A. Krissinger

Committee Member 1

Sara Neville Bennett

Committee Member 2

John W. Parrish, Jr.

Abstract

SS-129, the mutant in this study, is a new colonial mutant of the filamentous fungus, Neurospora crassamutant was isolated by Brown (1983) following ultraviolet irradiation of wild type conidia. SS-129 is termed "colonial" because growth of its mycelium is slow and restricted unlike the rapidly spreading mycelium of the wild type strain. The mutant is also characterized by inhibited growth at elevated temperature (37C), a failure to separate conidia, except in the presence of water, and a hydrophilic mycelium. Scanning electron microscopy revealed the colony of SS-129 as an elevated, dense fungal mass of extensively multidirectionally branched hyphae. Aerial conidial tufts develop from this mass. SS-129 advances by the formation of numerous peripheral growth fronts of overlapping hyphae which give the front a stacked appearance. Light microscopy revealed barren perithecia in crosses of SS-129 x SS-129. Genetic analysis of SS-129 indicated 1:1 Mendelian segregation of mutant and wild type progeny. Crosses to tester strains revealed linkage of SS-129 to the leu-4 and mating type loci in the left arm of Linkage Group I and nonallelism of SS-129 to the col-12 locus.

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