Term of Award
Master of Science in Biology
Document Type and Release Option
Thesis (restricted to Georgia Southern)
Department of Biology
Sara Neville Bennett
Committee Member 1
Wayne Allan Krissinger
Committee Member 2
Backcrosses of the osmotic-sensitive mutant SS-462 to wild type 74 indicated that the trait of osmotic sensitivity was due to a single gene under Mendelian control. A cross of SS-462 to the alcoy tester strain showed linkage to ylo-1, indicating that the locus for SS-462 was in LG III or LG VI.
SS-462 produced fewer conidia than wild type 74, but more than os-5. SS-462 did not differ from wild type 74, os-1, or os-5 in rate of hyphal elongation or in biomass.
The osmotic-sensitive mutant SS-462 failed to grow on medium with 2% NaCI and thus exhibited a high degree of sensitivity to that osmoticum. No evidence of sensitivity was seen when SS-462 was subjected to either KCI or glucose as osmotica. This, plus the fact that the morphology of SS-462 resembled that of wild type rather than that of typical osmotic-sensitive mutants, suggested that a mechanism other than, or in addition to, an altered cell wall might be responsible for the observed osmotic sensitivity of SS-462.
Mack, Katrina LaTrese, "Characterization and Genetic Analysis of SS-462, an Osmotic-Sensitive Mutant of Neurospora crassa" (1998). Legacy ETDs. 61.