Term of Award

Spring 1998

Degree Name

Master of Science in Biology

Document Type and Release Option

Thesis (restricted to Georgia Southern)

Department

Department of Biology

Committee Chair

Sara Neville Bennett

Committee Member 1

Wayne Allan Krissinger

Committee Member 2

Oscar Pung

Abstract

Backcrosses of the osmotic-sensitive mutant SS-462 to wild type 74 indicated that the trait of osmotic sensitivity was due to a single gene under Mendelian control. A cross of SS-462 to the alcoy tester strain showed linkage to ylo-1, indicating that the locus for SS-462 was in LG III or LG VI.

SS-462 produced fewer conidia than wild type 74, but more than os-5. SS-462 did not differ from wild type 74, os-1, or os-5 in rate of hyphal elongation or in biomass.

The osmotic-sensitive mutant SS-462 failed to grow on medium with 2% NaCI and thus exhibited a high degree of sensitivity to that osmoticum. No evidence of sensitivity was seen when SS-462 was subjected to either KCI or glucose as osmotica. This, plus the fact that the morphology of SS-462 resembled that of wild type rather than that of typical osmotic-sensitive mutants, suggested that a mechanism other than, or in addition to, an altered cell wall might be responsible for the observed osmotic sensitivity of SS-462.

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