Term of Award

Summer 1985

Degree Name

Master of Science

Document Type and Release Option

Thesis (restricted to Georgia Southern)

Committee Chair

Sara Neville Bennett

Committee Member 1

Donald J. Drapalik

Committee Member 2

Wayne A. Krissinger

Abstract

Chemical and morphological differences have been reported between the hyphal walls of wild type and osmotic mutants of Neurospora crassa. Rabbits were inoculated with isolated hyphal wall preparations from a wild type and an osmotically sensitive strain of Neurospora to determine if these chemical and morphological differences were also antigenically manifested.

Serum from the inoculated animals was found to contain antibodies specific for the hyphal wall. These attachment sites were found to occupy very specific locations on the hyphal surface, being localized at the septal and apical regions of hyphae, the interconidial septal furrows of proconidial chains, and interconidial connectives. It was found that antiserum raised to one strain would cross react with hyphal material from other strains. Additionally, tests utilizing immunoabsorbed serum indicated possible antigenic differences in the hyphal walls of strains differing in osmotic sensitivity, mating type and species, thus demonstrating the feasibility of using standard methods of immunological analysis to study the Neurospora hyphal wall.

Files over 10MB may be slow to open. For best results, right-click and select "Save as..."

Share

COinS