Term of Award

Spring 1994

Degree Name

Master of Science

Document Type and Release Option

Thesis (open access)

Committee Chair

Sara Neville Bennett

Committee Member 1

Wayne A. Krissinger

Committee Member 2

Donald J. Drapalik

Abstract

The mutant overaccumulator of carotenoids, ovc. was obtained from the Fungal Genetics Stock Center in Kansas City, Kansas. This mutant had been isolated following UV irradiation of the colonial mutant col-4 and mapped to Linkage Group IV (R. Harding, 1984 Neurospora Newsletter 34: 23-25). Examination of ovc in our laboratory revealed that in addition to having increased pigmentation, the mutant was osmotic sensitive, failing to grow on 6% NaCl. Morphology of ovc closely resembles that of cut, another osmotic sensitive mutant in LG IV. A cross of ovc to the osmotic sensitive mutant cut failed to produce recombinant progeny, supporting allelism of the two mutants. Reported location of ovc placed it between col-4 and met-5 in LG IV; however, cut is located to the far left of col-4. Examination of ovc gave no evidence of a translocation in the mutant.

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