Hepatic Transcriptomic Assessment of Sprague Dawley Rats in Response to Dietary Perfluorobutane Sulfonate (PFBS) Ingestion
Term of Award
Master of Science, Applied Physical Science
Document Type and Release Option
Thesis (open access)
Copyright Statement / License for Reuse
This work is licensed under a Creative Commons Attribution 4.0 License.
Department of Chemistry and Biochemistry
Dr. Worlanyo Eric Gato
Committee Member 1
Dr. Ji Wu
Committee Member 2
Dr. Amanda White
Perfluorobutane sulfonate (PFBS) is a short-chain PFAS that is considered to be a less toxic replacement for the rather more toxic long-chain perfluorooctane sulfonate (PFOS). Its numerous industrial applications and widespread presence in the environment have raised environmental and health concerns because of growing evidence associating adverse health effects and certain liver diseases to PFBS exposure. The study goal was to investigate whether dietary ingestion of PFBS would induce liver injuries, damage, inflammations, and oxidative stress. To achieve these goals, Sprague-Dawley rats were assigned into three PFBS dietary treatment groups (0, 50, and 100 PPM) for 11 weeks. After this period, the animals were sacrificed, and the serum and liver samples were evaluated for various clinical markers. There was a statistically significant increase (P < 0.05) in liver and body weights in the low-dose group relative to the control group. The Total Antioxidant Capacity (TAC) was significantly reduced (P < 0.05) in the PFBS-treated group relative to the control group. The study also revealed that serum ALT levels increased in a dose-dependent manner. Transcriptomic analysis showed 1,127 and 902 genes were differentially expressed in the low-dose and high-dose groups, respectively. Gene ontology analysis of differentially expressed genes showed that transmembrane transport and oxidation-reduction processes were the most significantly up-regulated biological processes when the control group was compared with the low-dose and high-dose groups, respectively. qRT-PCR results revealed that some pro-inflammatory genes (IL-7 and TNF-α) were up-regulated in the exposed group and those associated with oxidative damage (GPx1 and SOD1) were down-regulated.
Appiah, Isaac, "Hepatic Transcriptomic Assessment of Sprague Dawley Rats in Response to Dietary Perfluorobutane Sulfonate (PFBS) Ingestion" (2023). Electronic Theses and Dissertations. 2590.
Research Data and Supplementary Material
Available for download on Saturday, April 20, 2024