Modeling Post-Translational Modification in Cobalt Nitrile Hydratase with a Metallopeptide from Anne Jones

Document Type


Publication Date


Publication Title

Virtual Inorganic Pedagogical Electronic Resource


In this literature discussion, students read a paper about a cobalt metallopeptide that imitates the active site of the enzyme nitrile hydratase. Specifically, the model complex is oxidized by air to produce a coordination sphere with both cysteine thiolate and sulfinic acid ligands, much like the post-translationally oxidized cysteine ligands in the biological system. This paper also provides an introduction to a variety of physical methods used to characterize the structure, including X-ray absorbance spectroscopy, magnetic susceptibility using the Evans method, IR spectroscopy, electronic absorbance spectroscopy, and electron paramagnetic resonance spectroscopy. This LO was created for the 2014 TUES Viper Workshop on bioinorganic chemistry.