Method Development and Analyte Detection Using Liquid Chromatography Mass Spectrometry

Faculty Mentor

Nathaniel Shank

Location

Savannah Ballroom

Type of Research

On-going

Session Format

Poster Presentation

College

College of Science & Mathematics

Department

Biochemistry, Chemistry and Physics

Abstract

Liquid chromatography mass spectrometry (LCMS) is an analytical technique frequently used for the separation (LC) and detection (MS) of target analytes within a sample. Separation is based on the affinity of the analytes between a mobile phase and a stationary phase. In our research, we use high-performance liquid chromatography (HPLC), where analytes are dissolved in the liquid mobile phase and then pumped through a column containing the solid stationary phase. Each unique analyte establishes an equilibrium between the two phases which then dictates its elution time (how long it is retained in the column). Analytes exiting the column are then ionized and analyzed by the mass spectrometer. Detection can occur through “simple” scans but to accurately identify a target, we rely on the use of tandem mass spectrometry (MS/MS), a technique that fragments a known precursor ion and then detects a specified product ion. In our study, LC-MS/MS was utilized for the detection and quantification of specified analytes (tyrosine, perfluorooctanesulfonic acid (PFOS), variable mushroom compounds) in different samples. Our initial methods for these analytes were based on existing literature sources that we adapted and optimized for our instrument and running parameters. This report shows our initial results for these projects and our plans for further development and validation.

Program Description

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Start Date

4-21-2026 10:00 AM

End Date

4-21-2026 12:00 PM

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Apr 21st, 10:00 AM Apr 21st, 12:00 PM

Method Development and Analyte Detection Using Liquid Chromatography Mass Spectrometry

Savannah Ballroom

Liquid chromatography mass spectrometry (LCMS) is an analytical technique frequently used for the separation (LC) and detection (MS) of target analytes within a sample. Separation is based on the affinity of the analytes between a mobile phase and a stationary phase. In our research, we use high-performance liquid chromatography (HPLC), where analytes are dissolved in the liquid mobile phase and then pumped through a column containing the solid stationary phase. Each unique analyte establishes an equilibrium between the two phases which then dictates its elution time (how long it is retained in the column). Analytes exiting the column are then ionized and analyzed by the mass spectrometer. Detection can occur through “simple” scans but to accurately identify a target, we rely on the use of tandem mass spectrometry (MS/MS), a technique that fragments a known precursor ion and then detects a specified product ion. In our study, LC-MS/MS was utilized for the detection and quantification of specified analytes (tyrosine, perfluorooctanesulfonic acid (PFOS), variable mushroom compounds) in different samples. Our initial methods for these analytes were based on existing literature sources that we adapted and optimized for our instrument and running parameters. This report shows our initial results for these projects and our plans for further development and validation.