Term of Award

Spring 2022

Degree Name

Master of Science, Mechanical Engineering

Document Type and Release Option

Thesis (restricted to Georgia Southern)

Copyright Statement / License for Reuse

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 License.

Department

Department of Mechanical Engineering

Committee Chair

Mujibur Khan

Committee Member 1

Rafael Quirino

Committee Member 2

Anoop Desai

Abstract

Peptides have shown to have vasculature-homing properties with aminopeptidase N (CD13) which are also expressed on tumor vasculature. Two types of peptides, TAMS-1 U3012DA110-1CSPGAKVRCY{Lys(Biotin)} and Biotin{PEG4}-MEWSLEKGYTIK, were individually electrosprayed with a biodegradable polymer, polycaprolactone (PCL). The peptide solution was created by dissolving 2mg of the peptide into 50ml of DMSO. The PCL solution was created by dissolving PCL in a mixture of Acetic Acid (AA), Formic Acid (FA), and Trifluoroethanol (TFE) in a 9:9:1 ratio. The peptide was coaxially electrosprayed in two different trials (one trial for each peptide) with PCL, where PCL is the core and the peptide is the sheath. Electrospraying parameters included applying a voltage of 44kV, humidity between 35-44%, tip to collector distance at 160mm, core flow rate of 0.5 ml/hr, and a sheath flow rate of 0.7 ml/hr.

Development of a transverse axial spinneret was created to add another layer to the nanocapsule for a total of three layers. Samples were created using Fluorescein Sodium Salt as the core, PCL as the 1st sheath, and Rhodamine-6G as the 2nd sheath. Electrospraying parameters included applying a voltage of 43kV, humidity between 35-44%, tip to collector distance at 185mm, core flow rate at 0.1ml/hr with a 27 gauge needle and 5mL Becton plastic syringe, sheath flow rate for the 1st sheath at 0.2ml/hr using the sheath of the coaxial spinneret, sheath flow rate for the 2nd sheath at 0.2ml/hr with a 27 gauge needle and a 5mL Becton plastic syringe mounted onto the transverse spinneret.

Ultraviolet-visible (UV-VIS) spectrophotometry is used to detect peptides present in the solution before electrospraying and peptide presence in the nanoparticles after electrospraying. The UV-VIS detected the presence of the TAMS-1 peptide in the electrosprayed nanoparticle at a wavelength of 230nm-280nm.

Scanning Electron Microscopy (SEM) was completed to see the morphology of the active tumor targeting biodegradable/biocompatible polymer and the nanocapsules manufactured with three layers. Images showed individual nanocapsules sized at 100nm or less. Conglomerates are found to be 1µm or smaller

In vivo biodistribution tests were completed using the two sets of samples created from the two different peptides. TAMS-1 U3012DA110-1CSPGAKVRCY{Lys(Biotin)}, showed uptake to tumors when analyzing the in-vivo biodistribution tests. Nanocapsules electrosprayed with PCL and NGR Peptide, Biotin{PEG4}-MEWSLEKGYTIK did not show uptake to the tumors.

Research Data and Supplementary Material

Yes

Available for download on Tuesday, April 20, 2027

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