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Master of Science, Mechanical Engineering
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Thesis (restricted to Georgia Southern)
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Department of Mechanical Engineering
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Nanonization and encapsulation of cancer drug has been an effective way of making the drug injectable for EPR based passive targeted delivery. When cancer drugs are subjected to electrospray with high voltage like 30-45kV, it forms drug nanocrystal, which has same efficacy as the normal drug. UV-Vis is used to detect the presence of drug in the sample and later on, drug release pattern over the day is found through UV-Vis as well. The effect of spraying parameter on morphology of nanocrystals are investigated using Scanning Electron Microscope (SEM). On top of that, encapsulation of chemo drug into biocompatible polymer through coaxial electrospraying is performed. No specific drug compound like Florine (F) is found in the Electron Dispersive X-ray Spectrometry (EDS) result, which proves the encapsulation. Single drug loaded nanocapsules can consistently delivery drug in PBS media over 9 days. Besides, significant drug efficacy is found in cell viability and In-vitro drug efficacy test. In addition, multiple anti-cancer agents (drug and inhibitor) are encapsulated into a single core-shell nanostructure as combinational chemotherapy. These multidrug loaded nanocapsules can continuously deliver both drug and inhibitor simultaneously over a significant long time (2 weeks). In-vivo test, no adverse side effect is found in mice body after the treatment. Optical imaging test shows substantial cancer cell killing and meaningful reduction of metastasis, which endorses both the drug and inhibitor release in mice body. Most importantly, we reported that these nanocapsules can significantly increase the survival of cancer affected mice which is 29 days while all the control mice died within 24 days. Besides, IRDye tagged nanocapsules are synthesized through electrospraying process for investigating the biodistribution of nanocapsules in mice body. However, IRDye cannot withstand at high voltage during electrospraying and consequently, the presence of IRDye in tagged nanocapsules are not found in UV-Vis and Optical imaging test. On the contrary, IRDye can be tagged with block copolymer, PCL-PEG-NH2 through chemical coupling reaction. Dialysis tube with specific porosity is used to separate the unreacted IRDye from the tagged sample. PCL-PEG-NH2 tagged IRDye injected to C57BL/6 mice through tail vein in order to investigate the biodistribution. After 1 hour of injection, all the major organs like liver, lungs, heart, spleen, intestine and kidneys are tested in AmiX (includes X-Ray imaging) for ex-vivo analysis. Most prominent photon intensity is found at gastrointestinal tract and liver while least photon intensity is found at heart and brain.
Hasan, Md Mahmudul, ''Synthesis and Processing of Nanocapsules of Single and Multiple Cancer Drugs for Targeted Cancer Therapy" (2019). Electronic Theses and Dissertations. 3079. https://digitalcommons.georgiasouthern.edu/etd/3079
Research Data and Supplementary Material
Available for download on Friday, September 18, 2020