Analysis of Hepatic Protein Expression in Fisher-344 Rats Fed 2-Aminoanthracene

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Journal of Pharmacy and Biological Sciences






The effect of 2-aminothracene (2AA) ingestion in Fisher-344 rats was examined. Protein expression was examined via bulk proteins isolated from the liver and analyzed on a mass spectrometry. 2AA is an aromatic amine that can be classified as a polycyclic aromatic hydrocarbon (PAH). 2AA is used in the manufacture of chemicals, dyes, inks, and it is also a curing agent in epoxy resins and polyurethanes. 2AA has also been found in tobacco smoke and cooked foods.Total protein was extracted from liver tissues, separated via gel electrophoresis, trypsin-digested and analyzed using mass spectrometry. Mass spectrometric analysis of bulk hepatic proteins yielded a massive amount of proteins that seem to play varied roles in the maintenance of cellular homeostasis and integrity. The application of statistical algorithm to the dataset revealed the over expression of proteins that mediate energy related cellular and oxidative stress processes. Applying statistical algorithm to the bulk dataset reduced peptides to 28 common proteins that could be potential biomarkers of 2-aminoanthracene toxicity. Some of these peptides were Fetuin-B, Hemopexin, Pyrethroid hydrolase Ces2a and 2-hydroxyacyl-CoA lyase 1. Fetuin-b and hemopexin, which could be possible biomarkers of 2AA toxicity in the liver.Further analysis of these proteins via DAVID bioinformatics tool showed functional annotation terms related to oxidative stress responsive mechanisms, apoptosis related responses and various binding related processes such as monocarboxylic acid binding, cofactor binding and glutathione binding. Amount of quantified hepatic catalase concentration was found to be consistent to the bulk protein levels in the liver.