The Effect of Acute Exposure to TiO2 Nano Fiber in the Lungs of Sprague Dawley Male Rats

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In the past two decades, nanotechnology has grown tremendously. Synthetic nanomaterials have many unique chemical and physical properties, mainly due to their huge specific surface area and quantum confinement effect. Specifically titanium dioxide (TiO2) nanomaterial have high stability, anticorrosive and photocatalytic properties. These nanomaterials have applications for semiconductor photocatalysis, treatment of water or as a photoactive material in nanocrystalline solar cells. Recent studies show that TiO2 nanoparticle exposed to UV light irradiation show antibacterial properties and because of this they are under investigation for its use in nanomedicine. However, there are concerns over adverse effects resulting from bio-effects. The objective of the present study is to investigate the adverse effect associated with acute ingestion of TiO2 nanofiber (TDNF). TDNF was fabricated via electrospinning method, followed by dissolution in water. Six to seven weeks male Sprague Dawley rats were exposed to a total of 0, 40 and 60 ppm of TDNF for two weeks via oral gavage. Rats were euthanized via CO2 asphyxiation. Various organs including the lung was snap-fro-zen in liquid nitrogen. Sections of the tissues were also fixed in neutral buffered formalin for histopathological analysis. Preliminary morphometric data indicate slight reduction in body and lung weight. Measure of total protein also decreased in rats that ingested TDNF. Anatomical assessment of the pulmone indicate collapsed lung, bronchiolar arteriole hyperplasia, mild bronchiolar and alveolar hemorrhage, perivascular edema. The study is still ongoing to investigate genome-wide expression of transcripts in the lung.


Society of Toxicology Annual Meeting (SOT)


New Orleans, LA